Methods in Anopheles Research Manual
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Methods in Anopheles Research Manual
Preface
Table of Contents
Chapter 1 : Insectary Operation
1.1 Equipping and Operating an Insectary
1.2 Cleanliness and General Maintenance
1.3 Scheduling and Regulating Your Work Load
1.4 Maintaining Stock Purity
1.5 Insectary Manager Responsibilities
Chapter 2 : Anopheles Laboratory Biology and Culture
2.1 Behavior and Physiology of Anophelines in the Laboratory
2.2 Infections in Mosquito Cultures
2.3 Modifying Fecundity, Longevity and Size
2.4 Anopheles Culture
2.4.1 Bleaching Anopheles Eggs
2.4.3 Estimating the Number of Eggs and Larvae
2.4.4 Anopheles Larval Culture
2.4.5 Separating Larvae and Pupae
2.4.8 Bloodfeeding : Membrane Apparatuses and Animals
2.4.9 Collecting Anopheles Eggs
2.5 Basic Anopheles Mendelian Genetics
2.6 Basic Anopheles Population Genetics
Chapter 3 : Specific Anopheles Techniques
3.1 Determining the Sex of Anopheles Pupae and Larvae
3.2 Microinjection Methods for Anopheles Embryos
3.3 Plasmodium Sporozoite ELISA
3.4 Family Culture
3.5 Determining Egg Hatch Rates
3.6 Anopheles Mating
3.6.1 Mating : General Considerations
3.7 Anopheles Embryo Fixation
3.8 Mosquito Anesthesia
3.9 Eye Color Mutant Screening
Chapter 4 : Stock Authentication
4.1 Authentication by Morphological Characteristics
4.2 Authentication by PCR
4.2.1 Anopheles gambiae complex (Scott et al.)
4.2.2 Anopheles gambiae complex (Wilkins et al.)
4.2.3 Anopheles funestus complex
4.2.4 Anopheles minimus complex
4.2.5 Anopheles quadrimaculatus complex (Cornel et al.)
4.2.6 Anopheles quadrimaculatus complex (Levine et al.)
4.2.7 Anopheles gambiae Tep 1 Assay
4.2.8 Anopheles ITS2 Amplification
4.2.9 An. gambiae Ribosomal DNA Type (Fanello et al.)
4.2.10 An. gambiae Ribosomal DNA Type (Wilkins et al.)
4.3 Authentication by Bioassay
4.3.1 Larval Insecticide Resistance Assays
4.3.4 Microplate Enzyme Activity Assays Introduction
4.3.5 Microplate Insensitive Acetylcholinesterase Assay
4.3.6 Microplate Glutathione s-transferase Assay
4.3.7 Microplate Nonspecific Esterase Assay
4.3.8 Microplate Oxidase Assay
4.4 Insecticide Resistance Allele Assay by PCR
4.4.1 Knockdown Resistance - Anopheles gambiae
4.4.2 Dieldrin Resistance - An. gambiae and An. arabiensis
4.4.3 Knockdown resistance in Anopheles gambiae - Huynh et al.