Transgenic
|
|
Strain |
Organism |
MRA No. |
Notes |
Thumbnail |
P-BAC-0
|
A. gambiae
|
335
|
eGFP expression particularly in the
anterior stomach and distal lobes of salivary glands; Contains
pK[BIGalpha] insertion in 28D. See Genbank accession numbers AF402294
and AF402295
|
 |
IV
|
A. stephensi
|
314
|
L4s of this stock showed GFP expression
primarily in gastric cecae and the posterior stomach
|
 |
SRtTA
|
A. stephensi
|
728
|
SRtTA is
homozygous for Actin 5C-eGFP marker with an autosomal insertion of a
reverse tTA which is controlled by a serpin 10 promoter fragment.
Progeny crosses to a LacZ reponder line reveal staining in the midgut,
hemocytes and pericardial cells of larvae and adults.
|
 |
StTA/ tetOPLacZ
|
A. stephensi
|
727
|
This stock
has an X-linked tetracycline transactivator gene driver (tTA1) which is
controlled by a seprin 10 promoter fragment. Regulation of this system
is done via doxycycline. This strain is double marked with an Actin
5c-eGFP and an Actin 5C-dsRed1 marker. Lines carrying individual
insertions should be isolated following outcrossing to wild type A.
stephensi.
|
 |
B1
|
A. stephensi
|
855
|
RNA silencing of eGFP transcripts in
transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed
marker under Actin5C promoter. Parental crosses result in 43% reduction
in GFP expression. Backcrosses of progeny to B1 males results in 73%
reduction in expression.
|
 |
19.1
|
A. stephensi
|
854
|
RNA silencing of eGFP transcripts in
transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed marker
under Actin5C promoter. Parental crosses result in a 37% reduction in
GFP expression. Backcrosses of progeny to B1 males results in a 65%
reduction in expression.
|
 |
