Wild Stocks
|
|
Strain |
Organism |
MRA No. |
Origin |
Notes |
|
|
A. gambiae
|
112
|
Gambia
|
wild-type stock that has been in culture for over 30
years. It is considered a standard research organism, TEP1 s/s, G3 is primarily a
Savanna type stock
|
|
|
A. gambiae
|
186
|
Kenya
|
This strain was recently found to have a potential
translocation of the X chromosome onto the Y chromosome
|
|
|
A.
gambiae
|
763
|
Mali
|
This is a wild-type Mopti
rDNA form stock.
|
|
|
A. gambiae
|
762
|
Kenya
|
This strain is considered the insecticide susceptible
standard
|
|
|
A. stephensi
|
128
|
India
|
This strain is often used in Plasmodium berghei
studies
|
|
|
A. atroparvus
|
493
|
Spain
|
wild type
|
|
|
A. freeborni
|
130
|
United States
|
wild type, wild eye and body color, excellent
sporozoite-producer strain
|
|
|
A. farauti
|
489
|
Papua New Guinea
|
Infects well with most Plasmodium species.
An. farauti no. 1
|
|
|
A. arabiensis
|
339
|
Zimbabwe
|
This strain is considered a standard research
organism
|
|
|
A. arabiensis
|
856
|
Sudan
|
This strain was created for the SIT program in Sudan
|
|
|
A. quadrimaculatus
|
139
|
United States
|
wild type colony. An. quadrimaculatus A
|
|
|
A. albimanus
|
126
|
El Salvador
|
This stock was developed during the SIT program in El
Salvador
|
|
|
A. quadriannulatus
|
761
|
South Africa
|
wild
type, A. gambiae
complex member
|
|
|
A. dirus
|
700
|
Thailand
|
force mated species. A. dirus A
|
|
|
A. minimus
|
729
|
Thailand
|
wild type colony. A. minimus A
|
|
|
A. merus
|
803
|
South Africa
|
wild-type,
A. gambiae complex member
|
|
|
A. gambiae
|
861
|
Mali
|
This strain was used in the recent genome project (www.vectorbase.org)
and is the Savanna rDNA form standard
|
|
|
A. gambiae
|
860
|
Mali
|
This strain was used in the recent genome project (www.vectorbase.org)
and is the Mopti rDNA form standard
|
Transgenic
|
|
Strain |
Organism |
MRA No. |
Notes |
Thumbnail |
P-BAC-0
|
A. gambiae
|
335
|
eGFP expression particularly in the
anterior stomach and distal lobes of salivary glands; Contains
pK[BIGalpha] insertion in 28D. See Genbank accession numbers AF402294
and AF402295
|
 |
IV
|
A. stephensi
|
314
|
L4s of this stock showed GFP expression
primarily in gastric cecae and the posterior stomach
|
 |
SRtTA
|
A. stephensi
|
728
|
SRtTA is
homozygous for Actin 5C-eGFP marker with an autosomal insertion of a
reverse tTA which is controlled by a serpin 10 promoter fragment.
Progeny crosses to a LacZ reponder line reveal staining in the midgut,
hemocytes and pericardial cells of larvae and adults.
|
 |
StTA/ tetOPLacZ
|
A. stephensi
|
727
|
This stock
has an X-linked tetracycline transactivator gene driver (tTA1) which is
controlled by a seprin 10 promoter fragment. Regulation of this system
is done via doxycycline. This strain is double marked with an Actin
5c-eGFP and an Actin 5C-dsRed1 marker. Lines carrying individual
insertions should be isolated following outcrossing to wild type A.
stephensi.
|
 |
B1
|
A. stephensi
|
855
|
RNA silencing of eGFP transcripts in
transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed
marker under Actin5C promoter. Parental crosses result in 43% reduction
in GFP expression. Backcrosses of progeny to B1 males results in 73%
reduction in expression.
|
 |
19.1
|
A. stephensi
|
854
|
RNA silencing of eGFP transcripts in
transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed marker
under Actin5C promoter. Parental crosses result in a 37% reduction in
GFP expression. Backcrosses of progeny to B1 males results in a 65%
reduction in expression.
|
 |
